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Silencing of wcaG expression by antisense RNA. Expression levels of wcaG were determined by qRT-PCR; *wildtype-pHN680+IPTG vs. wildtype-pHN680, P < 0.05 (Student's t-test) (A). Growth of planktonic bacteria was measured by optical density at 600 nm (B). Biofilm formation of K. pneumoniae bacteremia strains was detected by crystal violet and the optical density determined at 550 nm (C). Errors bars represent mean ± SD or three replicates.
