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. 2018 Feb 23;9:322. doi: 10.3389/fmicb.2018.00322

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Copyright © 2018 Becker, Fuchs, Pfeifer, Semmler, Eckmanns, Korr, Sissolak, Friedrichs, Zill, Tung, Dohle, Kaase, Gatermann, Rüssmann, Steglich, Haller and Werner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gel electrophoresis of the cluster 1 specific PCR products (inverted image). PCR products (3 μl) were separated on 1.4% agarose gels. Isolates belonging to the major outbreak strain (cluster 1) are highlighted in green. (A) Clinical isolates from the neurorehabilitation clinic that were used to setup the PCR. (B) Further clinical isolates that occurred from June 2015 till April 2016 in the neurorehabilitation clinic, and were PCR-typed prior WGS. (C) Ten epidemiologically independent ESBL-producing (A–J) and carbapenemase-producing (K–T) K. pneumoniae isolates, respectively (see Table S3), used to check the specificity of the PCR. Interpretation: all three bands present: isolate belongs to cluster 1 (“outbreak strain”); all bands present but PCR product of 4,160 kb missing: isolate probably belongs to cluster 1; no PCR product or only amplification of the hem product: isolate does not belong to cluster 1.