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. 2018 Feb 27;8:3704. doi: 10.1038/s41598-018-21735-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dnm3os promoter is active in skeletal cell lines. (A) Schematic representation of Dnm3os gene (not scaled) and promoter region analysed (~850 bp). Putative promoter sequences of 8 vertebrates were aligned using CHAOS/DIALIGN, and then fed to ConTra v2: core match = 0.95, similarity matrix = 0.85, TRANSFAC database. TFBS predicted are indicated. Vertical lines indicate previously validated TFBS (by ChIP-assay) according to UCSC Genome browser. TSS, Transcriptional Start Site, based on human mRNA sequence; bp, base pairs. (B) Functional analysis of zebrafish dnm3os putative promoter activity in ABSa15 and ATDC5. Cells were transfected with zebrafish full (−1926 bp/+244 bp), partial (−657 bp/+244 bp), TATA-less (−1926bp/−140bp) promoter constructs, or promoter-less vector. (C) Functional analysis of human Dnm3os putative promoter activity in MC3T3 and ATDC5. Cells were transfected with human full (−2299 bp/+190 bp), partial (−641 bp/+190 bp), TATA-less (−2299bp/−30bp) promoter constructs, or promoter-less vector. For B) and C), a schematic representation of each construct and the respective putative TFBS are indicated on the left side. Values are the mean ± s.d. of at least 5 independent experiments; one-way Anova, ***p < 0.001.