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. 2018 Feb 27;8:3668. doi: 10.1038/s41598-018-22080-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of carbohydrate and lipid contents. S. acutus cultures were grown in TAP (+N) and TAP-N (−N) medium. (A) Carbohydrate contents. (B) Lipid contents. (C) Neutral lipid in −N culture monitored by nile red staining at 604 nm. Results are shown as means ± SD (n = 3). Scale bars represent 20 µm. *p < 0.05, **p < 0.01 (t-test).