Table 1.

Genes and primers used for qPCR analyses in D. chilensis.

Category	Accession	Candidate gene	5′-3′ Forward primer	5′-3′ Reverse primer	Amplicon length (bp)	Primer efficiency
Metabolism	MF771090	Pyruvate kinase (PK)	CTAGCATTGCCCAGACATC	CTGTGATTGGTGCTAGGTGC	113	1.14
	MF771093	Glycogen phosphorylase (GlyP)	CTGGTCTAGGAAATGGCGG	GTTCTTCTGTCTGCCACCC	112	1.2
	MF771092	Succinate dehydrogenase (SDH)	CTGCTTCTTGCCGATGTCTG	CCCATGGTTCTTGATGCTC	267	1.18
	MF771091	Citrate synthase (CS)	GATGTATGGAGGAATGCGAGG	CTGTGCTTGTGTAGGGATGTC	163	1.13
	MF771094	Alternative oxidase (AOX)	CCTGCACTACCATTCCTCC	CGTCTCGTCAATCATACCC	231	1.18
Defense system	MF695710	Glutathione synthetase (GSS)	GCTGGTAGTGTATGGAGGGAC	CGATGAAGACAGGAAGCTG	121	1.19
	MF771089	Glutathione peroxidase (GPX)	GGTCAATGGTCGATGCGTAG	GGGGAGGAGCTATTGAATGG	93	0.98
Stress response and damage cell	MF774028	HSP70	CTCCTGTTGTTGGATGTTGCC	GTTGTCTTTGGTCATGGCTC	148	1.1
	MF774029	HSP90	CCTTAATGCGCCTCTCTTCC	GATAGGACAGTTTGGTGTGGG	183	1.12
	MF774030	Laminin receptor	CAACCTGAAAAAGACCTGGG	GGGAGTCAGTATTGCACAAGG	270	1.07
Housekeeper	MF774031	Tubulin	GAACCATACAACGCCACC	GAAATGTAGACGTGGGAAGGG	216	1.18
	MF774032	Beta-Actin	CATTGTGGGAAGACCTAGGC	GATCTTCTCCATGTCGTCCC	117	1.09
	MF776702	EF1-alfa	GGACACGTAGACTCTGGAAAG	GTCTCAAACTTCCACAGGGC	156	1.12
	MF776703	RPL8	CTGTATGGAGGAGAAGGCTGG	GTTCATGGCCACACCTCTG	234	1.17