Table 1.
Experimental conditions of the CCD design in coded and natural variables and the corresponding observed and predicted responses.
| No. exp. | X1 | X2 | X3 | X4 | X5 | pH | Temperature (°C) | OMWW (%) | Inoculum size (%) | Incubation time (days) | BS production (mg/L) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Observed | Predicted | |||||||||||
| 1 | −1.0 | −1.0 | −1.0 | −1.0 | 1.0 | 6.0 | 20.0 | 5.0 | 0.5 | 8.0 | 45.32 | 44.62 |
| 2 | 1.0 | −1.0 | −1.0 | −1.0 | −1.0 | 10.0 | 20.0 | 5.0 | 0.5 | 2.0 | 88.40 | 89.81 |
| 3 | −1.0 | 1.0 | −1.0 | −1.0 | −1.0 | 6.0 | 40.0 | 5.0 | 0.5 | 2.0 | 40.00 | 39.66 |
| 4 | 1.0 | 1.0 | −1.0 | −1.0 | 1.0 | 10.0 | 40.0 | 5.0 | 0.5 | 8.0 | 114.04 | 114.66 |
| 5 | −1.0 | −1.0 | 1.0 | −1.0 | −1.0 | 6.0 | 20.0 | 15.0 | 0.5 | 2.0 | 27.08 | 25.34 |
| 6 | 1.0 | −1.0 | 1.0 | −1.0 | 1.0 | 10.0 | 20.0 | 15.0 | 0.5 | 8.0 | 52.40 | 53.62 |
| 7 | −1.0 | 1.0 | 1.0 | −1.0 | 1.0 | 6.0 | 40.0 | 15.0 | 0.5 | 8.0 | 720.80 | 721.47 |
| 8 | 1.0 | 1.0 | 1.0 | −1.0 | −1.0 | 10.0 | 40.0 | 15.0 | 0.5 | 2.0 | 88.00 | 96.58 |
| 9 | −1.0 | −1.0 | −1.0 | 1.0 | −1.0 | 6.0 | 20.0 | 5.0 | 2.5 | 2.0 | 28.40 | 30.67 |
| 10 | 1.0 | −1.0 | −1.0 | 1.0 | 1.0 | 10.0 | 20.0 | 5.0 | 2.5 | 8.0 | 288.40 | 288.64 |
| 11 | −1.0 | 1.0 | −1.0 | 1.0 | 1.0 | 6.0 | 40.0 | 5.0 | 2.5 | 8.0 | 161.60 | 160.09 |
| 12 | 1.0 | 1.0 | −1.0 | 1.0 | −1.0 | 10.0 | 40.0 | 5.0 | 2.5 | 2.0 | 73.44 | 73.04 |
| 13 | −1.0 | −1.0 | 1.0 | 1.0 | 1.0 | 6.0 | 20.0 | 15.0 | 2.5 | 8.0 | 150.40 | 154.49 |
| 14 | 1.0 | −1.0 | 1.0 | 1.0 | −1.0 | 10.0 | 20.0 | 15.0 | 2.5 | 2.0 | 72.40 | 77.60 |
| 15 | −1.0 | 1.0 | 1.0 | 1.0 | −1.0 | 6.0 | 40.0 | 15.0 | 2.5 | 2.0 | 51.28 | 54.72 |
| 16 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 10.0 | 40.0 | 15.0 | 2.5 | 8.0 | 78.80 | 78.21 |
| 17 | −1.0 | 0.0 | 0.0 | 0.0 | 0.0 | 6.0 | 30.0 | 10.0 | 1.5 | 5.0 | 90.12 | 88.10 |
| 18 | 1.0 | 0.0 | 0.0 | 0.0 | 0.0 | 10.0 | 30.0 | 10.0 | 1.5 | 5.0 | 48.56 | 43.24 |
| 19 | 0.0 | −1.0 | 0.0 | 0.0 | 0.0 | 8.0 | 20.0 | 10.0 | 1.5 | 5.0 | 26.32 | 22.30 |
| 20 | 0.0 | 1.0 | 0.0 | 0.0 | 0.0 | 8.0 | 40.0 | 10.0 | 1.5 | 5.0 | 92.32 | 94.01 |
| 21 | 0.0 | 0.0 | −1.0 | 0.0 | 0.0 | 8.0 | 30.0 | 5.0 | 1.5 | 5.0 | 75.20 | 73.57 |
| 22 | 0.0 | 0.0 | 1.0 | 0.0 | 0.0 | 8.0 | 30.0 | 15.0 | 1.5 | 5.0 | 127.88 | 126.17 |
| 23 | 0.0 | 0.0 | 0.0 | −1.0 | 0.0 | 8.0 | 30.0 | 10.0 | 0.5 | 5.0 | 79.40 | 84.84 |
| 24 | 0.0 | 0.0 | 0.0 | 1.0 | 0.0 | 8.0 | 30.0 | 10.0 | 2.5 | 5.0 | 56.08 | 51.30 |
| 25 | 0.0 | 0.0 | 0.0 | 0.0 | −1.0 | 8.0 | 30.0 | 10.0 | 1.5 | 2.0 | 14.56 | 16.88 |
| 26 | 0.0 | 0.0 | 0.0 | 0.0 | 1.0 | 8.0 | 30.0 | 10.0 | 1.5 | 8.0 | 128.04 | 124.16 |
| 27 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 8.0 | 30.0 | 10.0 | 1.5 | 5.0 | 53.16 | 53.49 |
| 28 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 8.0 | 30.0 | 10.0 | 1.5 | 5.0 | 53.24 | 53.49 |
| 29 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 8.0 | 30.0 | 10.0 | 1.5 | 5.0 | 52.80 | 53.49 |
| 30 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 8.0 | 30.0 | 10.0 | 1.5 | 5.0 | 52.94 | 53.49 |
| 31 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 8.0 | 30.0 | 10.0 | 1.5 | 5.0 | 50.86 | 53.49 |