Figure 2.

Bax and Bak regulate both apoptotic and necrotic cell death. (a) to (c) show the comparison of animals (WT, Bak^−/−, Bax^fl/flBak^−/−, and Bax^−/−Bak^−/− mice in this order) for (a) amounts of Bax, Bak, and Bim proteins by Western blots (n = 6 mice/group), (b) DNA fragmentation (n = 6 mice/group) and (c) amounts of RIP1 and RIP3 proteins by Western blots in control (“−” or clear bars)and compressed (“+” or shaded black bars) muscle tissues. All values are expressed as means ± standard error of mean. Pairwise comparison was performed with Student’s t-test, *P < 0.05, **P < 0.01. One-way analysis of variance (ANOVA) and Tukey’s post hoc test were used to compare multiple groups, ^#P < 0.05, ^##P < 0.01.