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. 2018 Feb 27;8:3692. doi: 10.1038/s41598-018-22049-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The involvement of actin microfilaments in the structural organization of the olfactory epithelium and the development of receptor cells in C. inermis. (a) Transverse section through OSNs and SCs (transmission electron microscopy); (b–e) confocal microscopy; staining for F-actin (FITC phalloidin, green); (b) Mosaic organization of actin microfilaments in the olfactory epithelium (3D reconstruction); profile of the apical section of a mature OSN marked by an arrow; (c) transmitted light mode (the ciliary layer is indicated with arrows); (d) Top view of the olfactory epithelium; three OSNs (marked by arrows) incorporated in an ordered fine-mesh network of actin microfilaments in the upper layer of the olfactory epithelium (3D reconstruction); (e) the profile in Fig. 5a with the expressed actin cases of OSN dendrites (3D reconstruction); OSNs marked by arrows. Notation: OSN – olfactory sensor neuron; сSC – ciliated supporting cell; sSC – secretory supporting cell. The blue arrow shows the direction to the surface of the olfactory epithelium.