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. 2018 Feb 27;8:3692. doi: 10.1038/s41598-018-22049-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Disappearance of actin microfilaments at the tips (marked by arrows) of three OSNs whose dendrites are attached to the OE surface, in C. inermis. Staining for F-actin (FITC phalloidin, green) and for nuclei (DAPI, blue); laser confocal microscopy; 3D reconstructions. (a) А view of the OSNs from the outer side of the epithelium; the actin-lacking tip of an individual cell is marked by an arrow; the large meshes of F-actin associated with the supporting cells are clear; actin microfilaments in the axial cylinders of the dendrites are partially preserved; elongated nuclear material, the bulk of which is inside the dendrites, is visible; (b) a view of the OSNs from the lamina propria of the olfactory mucosa. Notation: 1 – OSN dendrites that contact the surface of the olfactory epithelium; 2 – Profile of the apical section of a mature OSN; 3 – Profile of the apical section of the SC. The red arrow indicates the direction to the surface of the olfactory epithelium.