Figure 4.

Detection of the fusion gene sequence in cell-free DNA using specific probe and primer sets. A: The range of reproducibility of rt-PCR with the specific probe and primer sets was confirmed. Diluted serial tumor gDNA of 1-0.063 ng was used. The dotted line indicates an approximate curve (R² = 0.9742, P = 0.0018); B: cfDNA samples of a patient and 10 healthy volunteers were analyzed using rt-PCR. rt-PCR was performed in duplicate, and mean Ct values are indicated. Standard deviation was calculated from duplicate samples. cfDNA: Cell-free DNA.