Table 1.
Overview of primers and probe sequences for all PCR methods
| Primers (5’-3’) | Sequence | Product size (bp) |
| Reverse transcription PCR | ||
| SYT-sense | CCAGCAGAGGCCTTATGGA | 118 |
| SSX2-antisense | GCACAGCTCTTTCCCATCA | |
| Genomic PCR | ||
| SYT1-sense | GTAGTTTGACCGGCTGCAGAA | |
| SYT2-sense | GGTGGTCTGGTTTGTTCACC | |
| SYT3-sense | GCAAATGTTTATTGAGCAACCA | |
| SYT4-sense | GGGAGAAATTAAAAGGGTGGA | |
| SYT5-sense | CACCTGTGAAACCATCAGCA | |
| SYT6-sense | TTTTCTTTATGGATTATGCTTTTGG | |
| SYT7-sense | GCTTACTAGGAGTTTCATTGTAATTG | |
| SYT8-sense | CAGCCTGATAAACTGTATACC | |
| SYT9-sense | GATTTGAATGCGTGATCACAAG | |
| SYT10-sense | GATTGGATTCCAGACATTGTG | |
| SYT11-sense | GCCACCTTGGAATTTGTTAATG | |
| SYT12-sense | CTGATGATTGAAGAAACCGAG | |
| SSX2-1-antisense | ACGGAGAATCAGGGTTCTTTGG | |
| SSX2-2-antisense | TCAGTCTCCACACTGGCAAC | |
| SSX2-3-antisense | TCAAGGCAACATCCGACTCC | |
| SSX2-4-antisense | TGGTTTCCAGGGATAGAATGCT | |
| Real-time PCR | ||
| SYT-specific-sense | CCAGCAACAGTAGTTTACTTTCTATC | 106 |
| SSX2-specific-antisense | AAACATAGGGAGGCGACAAA | |
| SYT-SSX2-FAM probe-sense | ATA+CAA+T+C+CA+G+CAG1 |
1
+ C, +T, + G = LNA base.