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. 2018 Feb 27;8:3720. doi: 10.1038/s41598-018-22035-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Feeding dsRNA causes mortality in BMSB. Certified organic beans were inserted in 2 ml tubes containing 300 µl of nuclease-free water and 20 µg of dsRNA (20 µg of dsRNA in water was added to the tube on the second and third day). Fifteen 2^nd instar nymphs were fed on each bean for seven days. The mortality was recorded on the 7^th day, and the corrected percent mortality was calculated using Schneider-Orelli’s formula. Mean + S.E (n = 3) are shown. The asterisk reflects significant differences in mortality rate (ANOVA, Student-Newman-Keuls Method, P < 0.05).