Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 27;8:3720. doi: 10.1038/s41598-018-22035-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Knockdown efficiency determined by RT-qPCR in dsRNA fed BMSB. Twenty micrograms of dsRNA targeting IAP, SNF7, PPI, ATPase, GPCR or GFP (control) was fed to BMSB nymphs on each day for three days. On the fourth day, the nymphs were fed on beans. Total RNA was isolated on the 4th day after initiation of feeding dsRNA. The RNA was converted to cDNA, and the cDNA and gene-specific primers were used to quantify mRNA levels of IAP, SNF7, ATPase, PPI and GPCR using RT-qPCR. The 18S rRNA mRNA levels were used to normalize expression. The mean of relative mRNA levels and SE (n = 3–4) in dsGFP and dsIAP (A), dsSNF7 (B), dsPPI (C), dsATPase (D) or dsGPCR (E) fed nymphs are shown. A one-tailed t-test was used to compare the mean of a single variable.