Table 1.

Protein recovered in releasing buffer from pH 3–6

Releasing pH	Percent recovery from pH 6.0 immobilization	Percent recovery from pH 8.0 immobilization
pH 3	87.8 ± 5.9	99.8 ± 4.1
pH 4	68.8 ± 4.4	86.5 ± 5.3
pH 5	55.6 ± 5.5	84.1 ± 3.1
pH 6	41.8 ± 3.5	47.8 ± 4.1

The release rate and total amount of protein recovered are pH dependent with the highest recovery at pH 3 after 60 min. Error bars indicate standard deviation from triplicate measurements

Errors indicate standard deviation from triplicate measurements