Fig. 4.

The autoinhibitory HD blocks NAD⁺ binding to PARP-1. a ITC results where BAD (525 μM) was titrated into PARP-1 CAT WT and CAT ΔHD, both at 50 μM. b, c Same as in panel a but performed with benzamide as the titrant. d ITC results where BAD (525 μM) was titrated into full-length PARP-1 WT (40 μM) in the absence or presence of DNA (40 μM, 8 bp-duplex). e ITC results where BAD (525 μM) was titrated into full-length PARP-1 E763A/E766A/D770A (40 μM). f NMR experiment showing the signal obtained for BAD (20 μM) alone in green, BAD (20 μM) with PARP-1 (40 μM) in blue, and BAD (20 μM) with PARP-1 (40 μM) and DNA (40 μM, dumbbell DNA containing a central 1 nt-gap) in black