Table 2.
Average binding affinities (KD) determined by ITC
| Protein | Compound | KD (μM) | n |
|---|---|---|---|
| CAT WT | BAD | No binding | – |
| CAT ΔHD | BAD | 5.87 + /− 0.62 | 0.63 + /− 0.09 |
| CAT WT | Benzamide | 3.79 + /− 0.98 | 0.49 + /− 0.02 |
| CAT ΔHD | Benzamide | 3.21 + /− 0.59 | 0.39 + /− 0.01 |
| FL WT | BAD | No binding | – |
| FL WT with DNA | BAD | 1.81 + /− 0.54 | 1.11 + /− 0.29 |
| FL D766/D770A | BAD | 2.49 + /− 0.02 | 0.96 + /− 0.10 |
| FL E763/D766/D770A | BAD | 0.38 + /− 0.23 | 1.00 + /− 0.13 |
| FL WT | Benzamide | 2.32 + /− 0.79 | 0.88 + /− 0.01 |
| FL D766/D770A | Benzamide | 2.81 + /− 0.11 | 0.81 + /− 0.14 |
| FL E763/D766/D770A | Benzamide | 2.99 + /− 1.76 | 0.89 + /− 0.07 |
The KD and stoichiometry (n) values were obtained by averaging the results of two or three independent ITC experiments. The reported errors are the calculated standard deviations (representing the range of values for experiments repeated two times)