Figure 6.

Map and EspF interact with distinct tight junction regulatory proteins. (A,B) Confluent cultures of untransfected, EGFP-EspF or EGFP-Map cells were lysed in 1 × RIPA buffer and the supernatant was incubated with rabbit anti-GFP antibody overnight at 4 °C. Interacting proteins were immuno-precipitated by mixing with 50 µl of protein G-agarose beads for 4 hours at 4 °C. After washing, the beads were subjected to western blot analyses (n = 3). Blots were probed with indicated primary antibodies. Experiments were performed three times and representative blots are shown. UT: Untransfected. Full-length blots are presented in Supplementary Figure S7.