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. 2018 Jan 9;9(3):283–297. doi: 10.1007/s13238-017-0499-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Mitochondrial respiratory function of neural progenitor cells differentiated from MELAS-iPSCs. (A) Immunofluorescence analysis of neural progenitor markers in MELAS-iPSC-derived NPCs. Scale bar, 100 mm. (B) RFLP analysis and quantification of m.3243A>G heteroplasmy in MELAS-iPSC-derived NPCs. (C) OCR of the NPCs differentiated from wild-type hiPSCs, MELAS-iPSCs and targeted subclones. (D) Quantitative analysis of basal oxygen consumption, ATP production, maximal respiration and proton leak of NPCs (n = 3, error bars represent ±SEM; **P < 0.05)