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. 2017 Oct 12;9(5):1516–1529. doi: 10.1016/j.stemcr.2017.09.011

Figure 5.

Figure 5

NEUROG1 Knockdown in Proliferating Progenitors

(A–D) Hoechst- and EdU-labeled iMOP cells after transduction with (A) scrambled (scrb) shRNA (n = 3) or (B) Neurog1 shRNA (n = 3). Hoechst- and NucRed-labeled iMOP cells after transduction with (C) scrb shRNA (n = 3) or (D) Neurog1 shRNA (n = 3).

(E) Cell counts from scrb shRNA (n = 3) and Neurog1 shRNA (n = 3) transduced cultures in each field of view (FOV).

(F) Largest cross-sectional area of otospheres from scrb shRNA (n = 20 otospheres from 3 experiments) and Neurog1 shRNA transduced cultures (n = 20 otospheres from 3 experiments).

(G) Relative Neurog1, Cdk2, and NeuroD1 transcript levels in scrb shRNA (n = 3) or Neurog1 shRNA (n = 3) transduced cells.

(H) Western blot of NEUROG1, CDK2, and ACTB after transduction with scrb shRNA (n = 3) or Neurog1 shRNA (n = 3).

(I) Relative NEUROG1 (n = 3) and CDK2 (n = 3) levels from western blots.

Error bars denote ±SEM. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.