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. 2017 Oct 12;9(5):1516–1529. doi: 10.1016/j.stemcr.2017.09.011

Figure 7.

Figure 7

Neurog1 Overexpression and Knockdown in Differentiating Neurons

(A) EGFP expression and TUBB3 labeling of PB-T-EGFP + Dox cells (n = 3).

(B) Hoechst, EGFP and TUBB3 labeling of PB-T-Neurog1 – Dox cells (n = 3).

(C) EGFP expression and TUBB3 labeling of PB-T-Neurog1 + Dox cells (n = 3).

(D) Percent of TUBB3-positive cells in PB-T-EGFP + Dox (n = 3), PB-T-Neurog1 – Dox (n = 3), and PB-T-Neurog1 + Dox (n = 3) cells at different time points during differentiation.

(E) Relative transcript levels of NeuroD1 and Cdk2 in differentiating PB-T-EGFP + Dox (n = 3), PB-T-Neurog1 – Dox (n = 3), and PB-T-Neurog1 + Dox (n = 3) after 3 days of Dox induction.

(F and G) Hoechst-, NEUROD1-, and TUBB3-labeled differentiating iMOP cells transduced with (F) scrambled shRNA (n = 3) or (G) Neurog1 shRNA (n = 3).

(H) Percentage of NEUROD1, TUBB3, and NEUROD1/TUBB3 in scrambled (scrb) shRNA (n = 3) and Neurog1 shRNA (n = 3) transduced cells.

(I) Relative transcripts levels of Neurog1, NeuroD1, and Cdk2 after transduction with scrb shRNA (n = 3) or Neurog1 shRNA (n = 3).

Error bars denote ±SEM. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.