Figure 1.

Genomic Organization of the Nestin and Leptin Receptor Genes

(A) Nestin and (B) leptin receptor genes in mice, rats, and humans. The graphs were created based on recent data from both the National Center for Biotechnology Information (NCBI) (www.ncbi.nlm.nih.gov) and the UCSC Genome Browser (genome.ucsc.edu). The accession numbers for leptin receptor isoforms are: NM_146146.2 (mouse Lepr-B isoform, transcript variant 1, 19 exons), NM_001122899.1 (mouse Lepr-A, transcript variant 3, 19 exons), NM_012596.1 (rat Lepr-B, 19 exons), and NM_002303.5 (human LEPR-B, transcript variant 1, 20 exons), NM_001003679.3 (human LEPR-A, transcript variant 3, 20 exons), NM_001198689.1 (human LEPR-A, transcript variant 6, 19 exons), and NM_001198687.1 (human LEPR-C, transcript variant 4, 19 exons). Representative leptin receptor isoforms, transcript variant identification numbers, exon numbers, and tissue-specific expression patterns were briefly indicated in the right panel. Of note, the asterisk sign (^∗) indicates that mouse bone marrow (BM) stromal cells express the Lepr isoform b based on the existence of Lepr-Cre⁺ cells around sinusoids of the BM (Zhou et al., 2014) and the NLS-Cre cassette (in the Lepr-Cre transgene), which is inserted into the 3′ of the stop codon at exon 19 of the transcript variant 1 (DeFalco et al., 2001).

E or Ex, exon; ExN, exon numbers; FL, fetal liver; HET, hematopoietic tissues; I, intron; Iso, leptin receptor protein isoform; Lepr/LEPR, the leptin receptor gene; Leprot/LEPROT, leptin receptor overlapping transcript; Nes/NES, the gene coding for nestin; tv, transcript variant; P, promoter.