Figure 3.

Gene Regulation, Data Interpretation, and Integration

(A) Regulation of transgene at different molecular levels. Transgene reporter expression may or may not overlap with endogenous gene expression patterns. With regard to a transgene reporter activation, various experimental outcomes may be possible, which need to be confirmed by additional downstream assays (e.g., mRNA and protein expression).

(B) A scheme of data integration between mouse transgene reporter data and human epigenomic databases. Data from mouse genetic models may be directly translated and integrated into human BM biology given that they shared highly similar genomic structures and regulatory elements. Existing genomic and epigenomic databases can be also used to facilitate mouse data interpretation and help design humanized mouse models.