Table 1.
Primer pairs and product sizes for gene transcripts.
| Gene transcript∗ | Primer pair | Product size (bp) |
|---|---|---|
| CLDN5 [58] | Forward: 5′-GCAGCCCCTGTGAAGATTGA-3′ Reverse: 5′-GTCTCTGGCAAAAAGCGGTG-3′ |
106 |
| CTNNB1 [59] | Forward: 5′-AAAGCGGCTGTTAGTCACTGG-3′ Reverse: 5′-GACTTGGGAGGTATCCACATCC-3′ |
132 |
| DENV | Forward: 5′-GCAGATCTCTGATGAATAACCAAC-3′ Reverse: 5′-TTGTCAGCTGTTGTACAGTCG-3′ |
102 |
| EIF2AK2 [60] | Forward: 5′-GGAAAGCGAACAAGGAGTAAGG-3′ Reverse: 5′-CCAAAGCGTAGAGGTCCACT-3′ |
97 |
| GAPDH | Forward: 5′-AGCTGAACGGGAAGCTCACTGG-3′ Reverse: 5′-GGAGTGGGTGTCGCTGTTGAAGTC-3′ |
209 |
| IFITM1 [61] | Forward: 5′-ACTCCGTGAAGTCTAGGGACA-3′ Reverse: 5′-TGTCACAGAGCCGAATACCAG-3′ |
155 |
| IFN-β [62] | Forward: 5′-AAACTCATGAGCAGTCTGCA-3′ Reverse: 5′-AGGAGATCTTCAGTTTCGGAGG-3′ |
168 |
| IL-6 [63] | Forward: 5′-ATGAACTCCTTCTCCACAAGCGC-3′ Reverse: 5′-GAAGAGCCCTCAGGCTGGACTG-3′ |
628 |
| ISG15 [64] | Forward: 5′-GAGAGGCAGCGAACTCATCT-3′ Reverse: 5′-AGCATCTTCACCGTCAGGTC-3′ |
99 |
| JAM-3 [65] | Forward: 5′-CCCTGTCTGTAGAGTGCCGAAG-3′ Reverse: 5′-GAGCCTGCGTCATTGGAAGC-3′ |
258 |
| OCLN [66] | Forward: 5′-TGCATGTTCGACCAATGC-3′ Reverse: 5′-AAGCCACTTCCTCCATAAGG-3′ |
235 |
| PPIA | Forward: 5′-GGCAAATGCTGGACCCAACACAAA-3′ Reverse: 5′-CTAGGCATGGGAGGGAACAAGGAA-3′ |
355 |
| RSAD2 [67] | Forward: 5′-TGACGGAACAGATCAAAGCA-3′ Reverse: 5′-GCACCAAGCAGGACACTTCT-3′ |
174 |
| TBP [68] | Forward: 5′-GCCTCCCCCACCCCCTTCTTT-3′ Reverse: 5′-GCCACACCCTGCAACTCAACATCC-3′ |
106 |
| TNF-α [63] | Forward: 5′-TCTCGAACCCCGAGTGACAA-3′ Reverse: 5′-TGAAGAGGACCTGGGAGTAG-3′ |
181 |
| VE-cadherin [69] | Forward: 5′-GGGTTTTTGCATAATAAGCAGG-3′ Reverse: 5′-GCACCAGTTTGGCCAATATA-3′ |
149 |
| ZO-1 [70] | Forward: 5′-GAACGAGGCATCATCCCTAA-3′ Reverse: 5′-CCAGCTTCTCGAAGAACCAC-3′ |
218 |
∗References for primers sequences sourced from the literature are provided. For primer pairs designed in-house, products were confirmed by sequencing. B2M: β-2-microglobulin; CLDN5: claudin 5; CTNNB1: catenin-β1; DENV: dengue viral RNA; EIF2AK2: eukaryotic translation initiation factor 2-alpha kinase 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IFITM1: interferon-induced transmembrane protein 1; IFN-β: interferon-β; IL-6: interleukin-6; ISG15: interferon-stimulated gene 15; JAM-3: junctional adhesion molecule 3; OCLN: occludin; PPIA: peptidylprolyl isomerase A; RSAD2: radical SAM domain-containing 2 (viperin); TBP: TATA-binding protein; TNF-α: tumor necrosis factor-α; ZO-1: zonula occludens 1.