TABLE 1.
Yeast strains and plasmids used in this study
| Strain or plasmid | Relevant description | Reference or source |
|---|---|---|
| Strains | ||
| Y300 | MATa can1-100 ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 | 17 |
| MHY26 | Y300 dun1::HIS3 | This study |
| MHY363 | Y300 sml1::KAN | 22 |
| MHY365 | Y300 sml1::HIS3 mec1::HIS3 | This study |
| AXY2466 | Y300 sml1::HIS3 mec1::HIS3 rnr2::KAN pMH813 | This study |
| AXY2488 | Y300 sml1::HIS3 mec1::HIS3 rnr2::KAN pMH1653 | This study |
| XWY58 | Y300 clb5::KAN | This study |
| XWY61 | Y300 rnr2::KAN pMH1653 | This study |
| XWY65 | Y300 rnr2::KAN pMH800C | This stud |
| XWY77 | Y300 clb6::KAN | This study |
| XWY86 | Y300 wtm1::MYC-WTM1 rnr2::KAN pXW97 | This study |
| XWY88 | Y300 wtm1::MYC-WTM1 rnr2::KAN pMH1387 | This study |
| SLJ1386 | MATa bar1 cdc28-as1 | M. Winey |
| P211 | MATa ura3 lys2 cyh2 his3 leu2 bob1-1 cdc7Δ1::HIS3 | R. Sclafani |
| P235 | MATa ura3 lys2 cyh2 his3 leu2 bob1-1 cdc7Δ1::HIS3 dbf4Δ1::URA3 | R. Sclafani |
| RSY743 | MATa trp1 leu2 ade1 arg4 his3 1 his7 cyh2 | R. Sclafani |
| RSY755 | RSY743 clb5::ARG4 | R. Sclafani |
| RSY756 | RSY743 clb6::ADE1 | R. Sclafani |
| Plasmids | ||
| pRS314 | CEN TRP1 | 47 |
| pRS415 | CEN LEU2 | 47 |
| pRS424 | 2μm TRP1 | 47 |
| pXW80 | pRS424-PGAL1,10-GST-CLB5 | This study |
| pXW81 | pRS424-PGAL1,10-GST-CLB6 | This study |
| pXW82 | pRS424-PGAL1,10-GST-CDC28 | This study |
| pXW97 | pRS415-PRNR2-HA-rnr2(T5A) | This study |
| pMH762 | pRS424-PGAL1,10-GST | This study |
| pMH800C | pRS314-PRNR2-3×Myc-RNR2 | This study |
| pMH813 | pRS415-PRNR2-3×Myc-RNR2 | 48 |
| pMH1387 | pRS415-PRNR2-HA-RNR2 | This study |
| pMH1653 | pRS314-PRNR2-3×Myc-rnr2(T5A) | This study |