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. 2018 Jan 10;7(1):bio025734. doi: 10.1242/bio.025734

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Zebrafish lacking prion protein exhibited minor reductions in memory in an age-dependent fashion. Prp2^−/− fish showed a trend towards an age-dependent decline in familiar object preference with the object preference test. (A) Flowchart summarizing the sequence of events in the object preference test. (1) Fish were first habituated to a tank of the same size as the testing arena (the holding tank). (2) Fish were then netted and moved to the testing tank containing two identical objects (F) for the 10-min training phase. (3) Fish were then moved back to the holding tank for a 1- or 5-min period (memory retention interval), during which time one of the familiar objects (F) in the testing tank was replaced with a novel object (N). (4) Finally, fish were placed back into the testing tank for the 10-min object preference test. (B,C) Sample heat maps of 1-year-old prp2^+/+ fish and prp2^−/− fish that displayed object preference during the test phase. Top down view of the test tank, wherein fish can swim around the novel object (N) and/or the familiar object (F). Warm colours (yellows and reds) in the heat map indicate this individual fish spent more time near the familiar object, which was interpreted herein as indicating the fish remembered this object from its earlier training phase (see Materials and Methods and assumptions in Discussion). Scale bar: 3.5 cm (the approximate size of an adult zebrafish). (D,E) Sample heat maps of 3-year-old prp2^+/+ fish and prp2^−/− fish. This representative prp2^+/+ fish (D) exhibited familiar object preference during the test phase, while this example prp2^−/− fish (E) did not (quantified across multiple replicates below). (F) 1-year-old prp2^−/− zebrafish displayed familiar object preference following a 1-min retention interval, while the 1-year-old prp2^+/+ fish did not, as revealed by the D1 index of object preference (# indicates significant difference from 0 at P<0.05 using the Wilcoxon Signed Rank test; n=13 prp2^+/+ fish, n=28 prp2^−/− fish). (G) 3-year-old prp2^+/+ fish displayed familiar object preference after a 1-min retention interval while 3-year old prp2^−/− fish did not (D1 discrimination index, # indicates significant difference from 0 at P<0.05 using the Wilcoxon signed rank test; n=16 fish/genotype). (H) Zebrafish lacking prion protein (prp2^−/−) displayed a notable, though not statistically significant, reduction in familiar object preference when comparing between ages as measured by D1 (values replotted from Fig. 2F,G).