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. 2018 Jan 10;7(1):bio025734. doi: 10.1242/bio.025734

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Zebrafish lacking prp2 exhibited an age-dependent difference in object appraisal. 3-year-old prp2^−/− fish spent more time in close proximity to the novel object than 1-year-old prp2^−/− fish in the NOA test. (A) Amongst 1-year-old fish, there was no significant difference between genotypes (prp2^+/+ and prp2^−/−) in time spent in the object (centre) zone (n=14 prp2^+/+ fish, n=29 prp2^−/− fish). (B) Time spent in the object (centre) zone was significantly greater for the 3-year-old prp2^−/− fish than for the 3-year- old prp2^+/+ fish (*P<0.05 with one-tailed Mann–Whitney test, n=16 fish/genotype). (C) 3-year-old prp2 ^−/− fish spent a significantly greater period of time in the object (centre) zone than 1-year-old prp2^−/− fish (*P<0.05 with the Mann–Whitney test; values replotted from Fig. 4A,B).