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. 2017 Dec 6;7(1):bio029264. doi: 10.1242/bio.029264

Fig. 3.

Fig. 3.

Esrrb expression depends on LIF and Jak activity in reprogramming. (A) qRT-PCR analysis of pluripotent genes in reprogrammed cells transduced with retroviral 4F (OKSM) or 4F plus Nanog, with or without LIF cytokine at a 3-week time point. Values are relative to R1-ESC standard. Endogenous and total (endo- plus viral expression) Nanog (eNanog and tNanog, respectively) expression are also shown. Data are mean±s.d. from two independent biological repeats. **P<0.01. (B) Representative images of pre-iPSCs seeded into 24-well plates and treated with LIF, LIF plus Jaki, LIF plus Jaki/CHIR, LIFAb or LIFAb/CHIR at day 12 of reprogramming. Scale bar: 250 μm. (C) GFP+ colonies induced from pre-iPSCs treated as described in B were counted at day 12. Data are mean±s.d. from three independent experiments. **P<0.01. (D) Number of total colonies developed from pre-iPSC reprogramming as described in B at day 12. Data are mean±s.d. from three independent experiments. **P<0.01. (E) qRT-PCR analysis for Esrrb, Sox2, and Nanog expression in pre-iPSCs treated with Jaki, Jaki plus CHIR, LIFAb or LIFAb plus CHIR at reprogramming day 12. Values are relative to R1-ESC standard. Data are mean±s.d. from three independent experiments. **P<0.01.