Fig. 7.

Interaction between BAF60c and cardiac transcription factors. (A) GST-fused Baf60c associates with ³⁵S-labeled, in-vitro synthesized TBX5 and NKX2-5. (B) BAF60c associates with TBX5 through its N-terminal domain. Left: schemes representing serial deletion constructs of BAF60c. Right: mapping the BAF60c-associating domain with GST-fused TBX5. (C) BAF60c associates with full-length Myocardin and serial deletions. Left: schematic representation of Myocardin deletion constructs. The region at amino acids 328–554 of Myocardin was essential for association with Baf60c. (D) BAF60c enhances activation of MYOCD and MEF2c on the Myl1 luciferase reporter. Data are mean±s.e.m. Asterisks indicate significantly different values (P<0.05 by ANOVA) for individual conditions by t-test.