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. 2017 Dec 8;7(1):bio029777. doi: 10.1242/bio.029777

Fig. 3.

Fig. 3.

sur-6 gpr-1/2 (RNAi) embryos display abnormal centrosome numbers and cell divisions. (A) Analysis of embryonic lethality in various partial RNAi conditions. For sur-6(RNAi), L4 worms were grown on sur-6 feeding plates for 24 h at 23°C. For gpr-1/2(RNAi), L4 worms were grown on control feeding plates for 16 h at 23°C, then transferred to grp-1/2 feeding plates for 8 h at 23°C. For sur-6 gpr-1/2(RNAi), L4 worms were grown on sur-6 feeding plates for 16 h at 23°C, then transferred to sur-6 gpr-1/2 feeding plates for an additional 8 h at 23°C (n=8 mothers per condition and >50 F1 embryos per mother). (B) Number of centrosomes per cell in wild-type, sur-6(RNAi), gpr-1/2(RNAi) and sur-6 gpr-1/2(RNAi) embryos (mean±s.d.; n=10 embryos in each condition). All embryos contained only two centrosomes during pronuclear meeting (PNM), which occurs shortly after fertilization. 3-cell embryos were not counted, as they are transient in the wild-type condition and do not have visible centrosomes during that brief time. (C) Representative images from B. Cell outline is in magenta. Blebs were visible in the sur-6 gpr-1/2 (RNAi) embryos, but were not counted as cells (magenta asterisks). (D) Time-lapse imaging of a sur-6 gpr-1/2(RNAi) embryo expressing GFP::SPD-5. Blue arrowheads indicate abortive cytokinetic furrow ingression. Green arrowheads indicate successful furrow ingression. See also Movie 6.