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. 2018 Jan 15;7(1):bio030338. doi: 10.1242/bio.030338

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Morphological changes induced by T3 treatment in trβ crispants. (A) Uninjected control tadpoles and trβ crispants at pre-metamorphic stage (stage 52–54) were treated with or without 10 nM T3 for 3 days and morphological changes observed. After 3 days of T3 treatment, regression of gills was observed in all uninjected control tadpoles (n=44/44), whereas these morphological changes were not induced in most of the trβ crispants (n=51/54). In contrast, morphological change did not occur in the gills of both uninjected control tadpoles and trβ crispants without T3 treatment. (B) Hind limb development was induced by T3 treatment in all uninjected control tadpoles (n=44/44) and trβ crispants (n=54/54). N values represent the total numbers of tadpoles in three independent experiments.