FIGURE 1.

(A) Schematic representation of the glycine riboswitch locus. Glycine riboswitch aptamers 1 and 2 are depicted as boxes I and II; genes are depicted as gray arrows, pointing into the direction of transcription; terminators 1225 and 1227 as predicted by TransTermHP (Kingsford et al., 2007) are drawn as stem–loop structures, and transcripts detected by Northern blotting (Figure 4) are depicted as black lines. (B) Schematic representation of the riboswitch-luc2 fusion constructs: pFW11_glyluc2 consists of an 889 bp genomic fragment containing the predicted glycine riboswitch sequence and its 5′ flanking genomic region including the promoter region fused to luc2. pFW11_promoter_riboglyluc2 consists of an 588 bp fragment including the promoter region 5′ of ribogly directly fused to luc2.