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. 2018 Feb 20;9:200. doi: 10.3389/fmicb.2018.00200

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Copyright © 2018 Khani, Popp, Kreikemeyer and Patenge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SAF gene and cation efflux system gene expression are repressed in the presence of high glycine concentrations. (A) Relative expression of the SAF gene (Na+/Ala symp) and cation efflux system gene (cation efflux) in comparison to glycine riboswitch (ribogly) transcript abundance. GAS M49 strain 591 was grown 3 h in the presence of different glycine concentrations as indicated, n = 3. (B) The promoter region does not mediate glycine-dependent induction of luc2 expression. GAS M49 strain 591/ pFW11_promoter_riboglyluc2 were grown 3 h in the presence of different glycine concentrations as indicated, n = 3. Relative expression Data are presented as mean values ± standard deviation. Statistical significance was determined using the Student’s t-test. Differences between samples were expressed as “ns”: not significant, P ≥ 0.05 and ^∗∗∗∗P < 0.0001.