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. 2018 Jan 24;46(4):1984–1997. doi: 10.1093/nar/gky041

Figure 1.

Figure 1.

Role of RNase III in rRNA processing. (A) BioAnalyzer traces of RNA extracted from WT and select RNase III mutants. (B) Extensions of 23S rRNA at the 5′ and 3′ ends in ΔA0061ΔA2542ΔA0384. A diagram of the rRNA operon shows primers used to amplify cDNA made from WT and ΔA0384 and Δtriple strains. Controls using WT PCC 7002 DNA as a template (DNA), no template (X), and 2 log DNA ladder (L) are also shown. (C) Proposed rRNA species seen in the BioAnalyzer traces.The 23S peak (labeled as A) breaks into two fragments B (524 bases) and C (2289 bases). rRNA species from the triple mutant with 5′ and 3′ extensions are labeled with an asterisk.