Figure 3.

Heatmap of gene expression of coding sequences on pAQ3 in RNase III mutants compared to WT. Log₂ fold change data for each gene was clustered using Cluster 3.0 (hierarchical clustering, correlation uncentered, centroid linkage). Genes not meeting the FDR cutoff (<0.005) were given a value of zero. A schematic of the coding sequences on pAQ3 and their locations and orientations is shown. There was a significant difference in pAQ3 levels compared to a DNA spike-in are shown for WT and ΔA2542 (P < 0.05, Student's t-test, two-sided, equal variance, n = 4). Averages are shown with the error bars showing the standard deviation.