Table 5. Evaluation of genetic accessibility of B. breve strains with different plasmids (expressed as average of duplicate attempts) and impact of R/M systems. Results are expressed in CFUs/microgram of plasmid DNA.
| B. breve strain | E. coli strain | E. coli plasmid DNA | B. breve plasmid DNA | E. coli ER2796 (pNZ-M.Bbr52I) plasmid DNA | Plasmid | Active R/Ms |
|---|---|---|---|---|---|---|
| DRBB27 | ER2796 (Dam-) | 5.95 × 104 | 6.0 × 104 | - | pNZ8048 (Cm) | No |
| NRBB50 | ER2796 (Dam-) | 5.4 × 104 | 4.85 × 104 | - | pAM5 (Tet) | Partial R/M |
| NRBB01 | EC101 (Dam+) | 8.8 × 103 | 1.4 × 105 | - | pNZ8048 (Cm) | Yes |
| NRBB02 | EC101 (Dam+) | 2.01 × 105 | 2.8 × 105 | - | pAM5 (Tet) | Yes |
| NRBB02 | ER2796 (Dam-) | No transformants | - | - | pAM5 (Tet) | Yes |
| NRBB57 | EC101 (Dam+) | 5.37 × 104 | 1.03 × 106 | - | pDM1 (Spec) | Yes |
| NRBB52 | C29251 (Dam-/Dcm-) | 3.53 × 102 | 7.05 × 105 | 1.37 × 106 | pAM5 (Tet) | Yes |