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. 2018 Feb 15;9:152. doi: 10.3389/fpls.2018.00152

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Copyright © 2018 Wang, Song, Zhang, Wang and Kang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-expression of tae-miR164 and TaMAPK4 in Nicotiana benthamiana leaves. (A) β-glucuronidase (GUS) phenotypes observed by histochemical staining. (B) Quantitative detection of GUS activity in leaves inoculated with different recombinant vectors at different time points using a fluorospectrophotometer. EV, empty vector; 4-MU, 4-methyl-umbelliferyl- β-D-glucuronide. The results are presented as the means ± standard errors of three biological replications. Significant differences were determined using Student’s t-test: ^∗P < 0.05.