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. 2018 Jan 18;46(4):1601–1613. doi: 10.1093/nar/gkx1320

Figure 5.

Figure 5.

OEC Effects on Other Intracellular Membranes. (A). Lysotracker Uptake. Accumulation of Lysotracker Red in HeLa Luc 705 cells was measured after a 1.5 h exposure of cells to the indicated concentrations of various analogs. Means ± SE. N = 3. (B) UNC7938 vs Chloroquine. HeLa Luc705 cells were exposed to SSO623 and then treated with various concentrations of UNC7938 or chloroquine for 2 h and then tested for luciferase induction following the methods described in Figure 1. Means ± SE. N = 3. (C) OEC Effects on Mitochondrial Membrane Potential. Cells were incubated with various concentrations of UNC7938 or UNC5103 for 80 min. At this point TMRE was added to 500 nM and the incubation continued for 20 min. Cells were then rinsed in PBS, lysed in 0.2% TX100, and the accumulation of TMRE in the lysate was quantitated using a 96 well plate reader. FCCP is known to reduce the mitochondrial membrane potential and was used as a positive control. Means ± SE. N = 3.