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. 2017 Dec 8;46(4):1777–1792. doi: 10.1093/nar/gkx1229

Figure 3.

Figure 3.

AtU1A is a U1 snRNP-specific protein in planta. (A) AtU1A interacts with two other U1 snRNP-specific proteins, AtU1-70K and AtU1C in yeast. The pGBK (AtU1A) or pGAD (AtU1-70K and AtU1C) plasmids were co-transformed into yeast and plated on –leucine/-tryptophan/-histidine (-L-T-H) medium. The Empty vector pGAD and AtU1A with pGBK were co-transformed into yeast and used as a negative control. (B) BiFC assays of interactions between AtU1A and AtU1-70K, AtU1A and AtU1C. C-YFP is an empty vector. The different combinations of plasmids were transformed into tobacco epidermal cells and the YFP signals were detected with a confocal microscope. (C) RNA-IP analysis of the association of AtU1A protein with AtU1 snRNA or the pre-mRNA of AtU1A with diverse fragments. (D) AtU1A does not interact with itself in yeast. The BD-AtU1A in pGBK and AD-AtU1A in pGAD plasmids were co-transformed into yeast and plated on –leucine/-tryptophan/-histidine (-L-T-H) medium. The empty vector pGAD and AtU1A in pGBK were co-transformed into yeast and served as a negative control. Asterisks indicate significant differences (***P < 0.001) as determined by a two-tailed paired Student's t-test.