Figure 6.

CFX riboswitch controls yeast growth. (A) Serial dilution growth assay were performed for both, 10AΔAUG (ΔAUG) and the engineered CFX riboswitch (CFX-RS). Additionally, two controls expressing the aminoglycoside 3′-phosphotransferase (kanR+) and a mutant without start codon ATG (kanR-) were spotted on SCD-ura plates (supplemented with G148) in the absence or presence of 1 mM CFX. Ten-fold serial dilutions were spotted from left to right (numbering above). Cells were grown for two days at 30°C. (B) Similar to the experiments with kanR, an analogous approach was performed by exchanging the kanR gene with URA3. Selection marker for plasmid maintenance were swapped.