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. 2018 Jan 9;46(4):1927–1944. doi: 10.1093/nar/gkx1315

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — (A) Schematic of the pGL3s-hRLuc construct. Upon translation of the dual luciferase mRNA two protein products were produced, a 36 kDa termination product (RLuc only) and a 102 kDa readthrough product (RLuc:Luc⁺ fusion). (B) Heat map of mean measured readthrough efficiency for 192 constructs (all possible permutations of the +4 to +6 positions for each stop codon). Top left quadrant, UAA; top right quadrant, UAG; bottom left quadrant, UGA. ‘+2’ and ‘+3’ refer to the identity of the second and third bases in the stop codon; ‘+4’, ‘+5’ and ‘+6’ refer to the three nucleotides immediately 3′ to the stop codon. Data are available in Supplementary Table S1.