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. 2018 Jan 9;46(4):1927–1944. doi: 10.1093/nar/gkx1315

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Termination efficiencies of the ⁺¹UGACUUNNN⁺⁹ signal (+7 to +9 positions). (A) Termination efficiencies of representative high and low efficiency ⁺¹UGACUUNNN⁺⁹ sequence contexts measured in COS-7 cultured cells. Readthrough (%) was calculated by comparison to a near-cognate control construct (UGG). Termination efficiencies are presented according to the identity of the fourth base following the termination codon (+7). Results are graphed relative to the median termination efficiency for the series (1.41%). Presentation format, numbers of replicates and error bars are as in Figure 2. Means not sharing the same letter are significantly different (Tukey HSD, P < 0.01). (B) The same data as in (A) but ordered according to the identity of the +8 nucleotide.