Figure 6.

Effect of regulation of eRF1 expression on termination efficiency. Termination efficiencies of ⁺¹UGANUG⁺⁶ (A) and ⁺¹UGACUN⁺⁶ (B) sequence contexts in HEK 293T cultured cells co-transfected with either the control plasmid pcDNA or the pcDNA-eRF1 over-expression plasmid (eRF1) (upper panels), or the shRNAs pSilencer negative control (NC), pSilencer-si90 or pSilencer-si1186 (lower panels). Readthrough (%) was calculated by comparison to a near-cognate control construct (UGG). GUG result is expanded in inset in left lower panel. Experiments were performed in triplicate with 12 replicates for each condition, and 24 replicates for pSilencer NC. Mean values are presented. Error bars are SEM. *P < 0.05, ***P < 0.01, n.s., not significant. Data are available in Supplementary Table S3.