Figure 7.

TFL2 is important for TFIIB recruitment to and retention at the SLRNA promoter. Anti-TFIIB ChIP assays were carried out with chromatin prepared from uninduced cells or cells in which TFL2 was silenced for three days. On the left, semi-quantitative PCR of the SLRNA promoter and part of the tubulin coding region in DNA prepared from total chromatin (input) and precipitate (IP). On the right, corresponding qPCR analysis from four independent experiments in which the TFIIB occupancy of the SLRNA promoter was normalized by that of the ATUB occupancy and the fold enrichment over input was calculated. The difference in TFIIB occupancy between uninduced and TFL2-silenced cells was statistically analyzed by an unpaired, two-tailed Student's t-test assuming equal variance.