TABLE 1 .
Strain list
| Strain | Relevant genotype |
|---|---|
| YJF153a | MATa HO::dsdAMX4 |
| DMy13 | mmf1::kanMX (ρ0) |
| DMy16 | cha1::hphMX |
| DMy17 | mmf1::kanMX cha1::hphMX (ρ0) |
| DMy18 | ilv1::natMX |
| DMy20 | cha1::hphMX mmf1::kanMX |
| DMy23 | YJF153 (ρ−) |
| DMy21 | mmf1::hphMX-loxP (ρ0) |
| DMy22 | mmf1Δ0 (ρ0) |
| DMy31 | YJF153/pDM1481 |
| DMy32 | YJF153/pSF-empty |
| DMy33 | mmf1Δ0 (ρ0)/pDM1481 |
| DMy34 | mmf1Δ0 (ρ0)/pSF-empty |
| DMy35 | YJF153 (ρ−)/pDM1481 |
| DMy36 | YJF153 (ρ−)/pSF-empty |
| DMy41 | mmf1::loxP-kanMX-loxP (ρ+) |
| DMy43 | ilv1Δ0::ILV1-1b |
| DMy46 | ilv1Δ0::ILV1-1 mmf1::kanMX |
| DM15531 | E. coli BL21-AI/pDM1463 |
| DM15533 | E. coli BL21-AI/pDM1467 |
| DM15910 | E. coli BL21-AI/pDM1536 |
a
All yeast strains were constructed in a YJF153 strain background.
b
Allele ILV1-1 encodes an Ilv1pR416F feedback-resistant variant.