FIG 5 .

Physically released merozoites are invasive. (A) Schematic of the method to generate viable free merozoites efficiently from [+] Shld-1 and [−] Shld-1 schizonts. 28G, 28-gauge; HCT, hematocrit. (B) Ring parasitemia from physically released [+] or [−] Shld-1 schizonts. The ring parasitemia was not different between the [+] and [−] Shld-1 conditions (n = 3; mean ± SD; no significant difference by Student’s t test). (C) Wide-field IFA of PfAMA1 localization before and after shearing from [+] and [−] Shld-1 parasites. Treatment of schizonts with 2.5 µM C1 prior to shearing prevents PfAMA1 translocation.