Table 1. Plasmids used in this study.
Genes are human unless otherwise stated
| Plasmid | Backbone | Insert | Ref |
|---|---|---|---|
| APX1-GFP1-10 | APX1 | super-folder GFP | This study |
| CDV-hyPBase | pigg | piggyBAC transposase | (Yusa et al., 2011) |
| NES-EGFP-P4M × 1 | pEGFP-C1 | X.leavis map2k1.L(32-44):EGFP:L. pneumophila SidM(546-647) | This study |
| FKBP-mCherry | pmCherry-N1 | FKBP1A(isoform a, 3–108):mCherry | This study |
| SAC1∆TMD-FKBP-mCherry | pmCherry-N1 | SACM1L(1-521):FKBP1A(3-108):mCherry | This study |
| SAC1C389S∆TMD-FKBP-mCherry | pmCherry-N1 | SACM1L(C389S; 1–521):FKBP1A(3-108):mCherry | This study |
| iRFP-Sec61β | piRFP-C1 | iRFP:SEC61B | This study |
| mKO-ManII | pmKO-N1 | Kusabira Orange 2:Man2a(1-102) | Tamas Balla |
| mCherry-VAPB | pmCherry-C1 | mCherry:VAPB | This study |
| mCherry-MAPPER | pmCherry-C1 | mCherry:MAPPER | (Chang et al., 2013) |
| EGFP-MAPPER | pEGFP-C1 | EGFP:MAPPER | (Chang et al., 2013) |
| GFP-ORP5 | pEGFP-C1 | EGFP:OSBPL5(isoform a) | (Sohn et al., 2016) |
| GFP-E-Syt2 | pEGFP-C1 | EGFP:ESYT2 | (Giordano et al., 2013) Addgene plasmid #66831 |
| EGFP-SAC1 | pEGFP-C1 | EGFP:SACM1L | (Sohn et al., 2016) |
| mEmerald-N16-Calreticulin | pmEmerald-N1 | mEmerald:CALR | Michael Davidson (Addgene plasmid #54023) |
| GFP-Sec61β | pAcGFP-C1-Sec61β | Aequorea coerulescens GFP:SEC61B | (Voeltz et al., 2006) Addgene plasmid #15108 |
| EGFP-E-Syt1 | pEGFP-C1 | EGFP-ESYT1 | (Giordano et al., 2013) Addgene plasmid #66830 |
| EGFP-STIM1 | pEGFP-C1 | STIM1(isoform 1 1–22):EGFP:STIM1(23–791) | (Várnai et al., 2007) |
| EGFP-Nir2 | pEGFP-N1 | EGFP:PITPNM1(isoform 2) | (Kim et al., 2015) |
| EGFP-P4M × 2 | pEGFP-C1 | EGFP:L. pneumophila SidM(546-647):SidM(546-647) | (Hammond et al., 2014) |
| Lyn11-FRB-iRFP | piRFP-N1 | LYN(1-11):MTOR(2021–2113):iRFP | (Hammond et al., 2014) |
| mCherry-FKBP | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG | (Hammond et al., 2014) |
| mCherry-FKBP-SAC1 | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L | This study |
| mCherry-FKBP-SAC1C389S | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S | This study |
| mCherry-SAC1-FKBP | pmCherry-C1 | mCherry:SACM1L:FKBP1A(3-108) | This study |
| mCherry-SAC1C389S-FKBP | pmCherry-C1 | mCherry:SACM1LC389S:FKBP(3-108) | This study |
| SAC1∆452-587-FKBP-mCherry | pmCherry-N1 | SACM1L(1-451):FKBP1A(3-108):mCherry | This study |
| mCherry-FKBP-SAC1∆TMD | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) | This study |
| mCherry-FKBP-SAC1-HLx2 | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) | This study |
| mCherry-FKBP-SAC1-HLx4 | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]4:SACM1L(521-587) | This study |
| mCherry-FKBP-SAC1-HLx6 | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]6:SACM1L(521-587) | This study |
| mCherry-FKBP-SAC1-HLx8 | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) | This study |
| mCherry-FKBP-SAC1-HLx10 | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) | This study |
| mCherry-FKBP-SAC1C389S-HLx8 | pmCherry-C1 | mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) | This study |
| mCherry | pmCherry-C1 | mCherry | (Hammond et al., 2014) |
| mCherry-SAC1 | pmCherry-C1 | mCherry:SAC1ML | (Sohn et al., 2016) |
| mCherry-SAC1-HLx8 | pmCherry-C1 | mCherryLSACM1L(1-520):[EAAAR]8:SACM1L(521-587) | This study |
| mCherry-SAC1C389S-HLx8 | pmCherry-C1 | mCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) | This study |
| mTagBFP2-FKBP-CYB5Atail | pmTagBFP2-C1 | mTagBFP2:FKBP1A(3-108):[GGSA]4GG:CYB5A(100-134) | This study |