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. 2017 Oct 12;9(5):1573–1587. doi: 10.1016/j.stemcr.2017.09.009

Figure 4.

Figure 4

Co-binding of Histone H3K27 Methyltransferase/Demethylase and Histone Acetyltransferase/Deacetylase to Gene Promoters

(A–D) Binding of the H3K27 methyltransferase EZH2 (A), the H3K27 demethylase UTX (B), the histone deacetylase HDAC1 (C), and the histone acetyltransferase p300 (D) to indicated gene promoters was measured by ChIP-qPCR. ChIPs were performed using indicated antibodies (Ab) or IgG as a negative control (Mock). qPCR values are expressed as mean fractions of input ±SD.

(E) Model representing co-binding of antagonistic enzymes on gene promoters such that transcription is maintained at low levels (poised gene). Combined inhibition of the repressive enzymes EZH2 and HDAC1 displaces the equilibrium leading to transcriptional activation.