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Cells were encapsulated in alginate beads separately and alginate and pellet co-cultures (A, control conditions). Furthermore, hMSCs and bACs were co-cultured in (B) a Transwell® system as well as in (C) medium conditioned by the other cell type, to further understand the complex cellular communication pathways between hMSCs and bACs. In purple: hMSCs = human Mesenchymal Stem Cells; in green: bACs = bovine Articular Chondrocytes.
