. 2018 Feb 28;13(2):e0190744. doi: 10.1371/journal.pone.0190744

Table 2. Sequences of primers for qRT-PCR.

Primers and probes
Human specific genes
*hsGAPDH*	Fw: `AGCTCACTGGCATGGCCTTC`
	Rev: `CGCCTGCTTCACCACCTTCT`
*hsACAN*	Fw: `CAGCCACCACCTACAAACGCAG`
	Rev: `CTGGGTGGGATGCACGTCAGC`
*hsCOL2A1*	Fw: `ACGAGGCCTGACAGGTCCCA`
	Rev: `GCCCAGCAAATCCCGCTGGT`
Bovine specific genes
*bsGAPDH*	Fw: `GTCAACGGATTTGGTCGTATTGGG`
	Rev: `TGCCATGGGTGGAATCATATTGG`
*bsACAN*	Fw: `GGACACTCCTTGCAATTTGAGAA`
	Rev: `CAGGGCATTGATCTCGTATCG`
*COL2A1*	Fw: `GGCAATAGCAGGTTCACGTACA`
	Rev: `CGATAACAGTCTTGCCCCACTT`

GAPDH = GlycerAldehyde 3-Phosphate DeHydrogenase; ACAN = Aggrecan; COL2A1 = Collagen type 2; hs = human-specific; bs = bovine-specific.