Fig. 5.

Effects of TINO with or without RT in patient derived glioma stem-like cells. a Effects of RT (0, 2, 4, and 6 Gy) with or without TINO in BT12M cell model. b DRE values calculated for BT12M, BT48EF, BT50EF, and CSCs-5 patient-derived glioma stem-like cells. c, d Western blot and ELISA determinations indicating the shift from authophagy to apoptosis. e DNA ladder in representative BT12M cells. f Table summarizing subG1 cell percentage measured with propidium iodide (PI) by using Tali™ instrument (Thermo Fisher Scientifics, Carlsbad, CA, USA) in BT12M, BT48EF, BT50EF, and CSCs-5 patient-derived glioma stem-like cells. g Caspase 3 activity performed in BT12M, BT48EF, BT50EF, and CSCs-5 patient-derived glioma stem-like cells. h Annexin V/PI-positive apoptotic cells performed by using Tali™ Apoptosis Kit - Annexin V Alexa Fluor™ 488 & Propidium Iodide. Single results are representative of three different experiments performed in triplicate